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  • Title: Allelic Discrimination by TaqMan-PCR for Genotyping of Human Neutrophil Antigens.
    Author: Steffensen R, Baech J, Nielsen KR.
    Journal: Methods Mol Biol; 2015; 1310():205-12. PubMed ID: 26024637.
    Abstract:
    Neutrophil antigens are implicated in a variety of clinical conditions, including neonatal immune neutropenia, transfusion-related acute lung injury, refractoriness to granulocyte transfusions, febrile transfusion reactions, and autoimmune neutropenia. In this report, we describe simultaneous genotyping of human neutrophil antigens (HNA)-1, -3, -4, and -5 using PCR with allele-specific TaqMan probes and end-point fluorescence detection, which is a robust, rapid, and reproducible method, allowing for high-throughput genotyping.
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