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  • Title: [Affinity modification of DNA-dependent RNA-polymerase from phage T7 with 5'-p-fluorosulfonylbenzoyl adenosine: the effect of modification on the interaction with substrates].
    Author: Tunitskaia VL, Luchin SV, Memelova LV, Liakhov DL, Rechinskiĭ VO, Kochetkov SN.
    Journal: Mol Biol (Mosk); 1989; 23(5):1273-8. PubMed ID: 2608036.
    Abstract:
    T7 RNA polymerase, covalently modified with 5'-p-fluorosulfonylbenzoyl adenosine, looses the ability of binding the promoter (pGEM-2 plasmid) and poly(dC) template as well as the initiating nucleoside triphosphate (GTP). However the enzyme retains the unspecific binding with DNA fragments of considerable length.
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