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  • Title: Molecular characterization of the Tobacco rattle virus RNA2 genome isolated from Gladiolus.
    Author: Kim YJ, Lim MS, Kim SM, Ryu KH, Choi SH.
    Journal: Acta Biol Hung; 2015 Jun; 66(2):222-30. PubMed ID: 26081277.
    Abstract:
    Tobacco rattle virus (TRV-K) was first identified in a symptomatic Gladiolus plant cultivated in Korea. We analyzed the TRV-K genome and compared its phylogeny with other TRV isolates. After constructing of a full-length genomic RNA2 strand clone, a complete sequence was generated from several overlapping clones. The cloned genome was 3261 bases in length, identical to TRV-K, and had three open reading frames. TRV-K had the highest sequence identity with the American isolate TRV-ORY. Sequence analysis of the RNA2 genome showed that TRV-K contains an intact 2a, 2b, and 2c coding sequence and an RNA1-related 3' terminus, which is typical of TRV RNA2. Phylogenetic analysis revealed that TRV-K is in the same cluster as the American isolates and another Korean isolate, TRV-SK; however, it was in a different cluster than the European isolates.
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