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  • Title: The synthesis and characteristics of adenylyl-(5'----N epsilon)-lysyl peptides.
    Author: Juodka B, Sasnauskiene S.
    Journal: Nucleic Acids Symp Ser; 1989; (21):137-8. PubMed ID: 2608464.
    Abstract:
    The dipeptides Boc-Thr-Lys(Cbz)-OMe and Boc-Lys(Cbz)-Glu(OEt)-OEt were prepared by the classical method of peptide synthesis. The Cbz protecting group was subsequently removed via hydrogenolysis. AMP was then covalently joined to the free lysine epsilon-amine by the carbodiimide (DCC) method. The resulting RNA-ligase active center model compounds, the adenylyl-(5----N epsilon)-lysylpeptides were then used to study the participation of the amino acid residue functional groups in the hydrolysis of the phosphoamide center.
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