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  • Title: Capillary electrophoretic studies on quantum dots and histidine appended peptides self-assembly.
    Author: Wang J, Li J, Chen Y, Teng Y, Wang C, Li J, Liu L, Dong B, Qiu L, Jiang P.
    Journal: Electrophoresis; 2015 Oct; 36(19):2419-24. PubMed ID: 26084876.
    Abstract:
    Herein, we designed four peptides appended with different numbers of histidine (Hisn -peptide). We launched a systematic investigation on quantum dots (QDs) and Hisn -peptide self-assembly in solution using fluorescence coupled CE (CE-FL). The results indicated that CE-FL was a powerful method to probe how ligands interaction on the surface of nanoparticles. The self-assembly of QDs and peptide was determined by the numbers of histidine. We also observed that longer polyhistidine tags (n ≤ 6) could improve the self-assembly efficiency. Furthermore, the formation and separation of QD-peptide assembly were also studied by CE-FL inside a capillary. The total time for the mixing, self-assembly, separation, and detection was less than 10 min. Our method greatly expands the application of CE-FL in QDs-based biolabeling and bioanalysis.
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