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  • Title: Aptamer-functionalized nanoporous gold film for high-performance direct electrochemical detection of bisphenol A in human serum.
    Author: Zhu Y, Zhou C, Yan X, Yan Y, Wang Q.
    Journal: Anal Chim Acta; 2015 Jul 09; 883():81-9. PubMed ID: 26088780.
    Abstract:
    In the present work, a highly sensitive and selective biosensor based on aptamer-functionalized nanoporous gold film (NPGF) was successfully developed for direct electrochemical detection of bisphenol A (BPA). NPGF was prepared by dealloying Ag from Au/Ag alloy leaf in concentrated nitric acid. The obtained NPGF was attached onto glassy carbon electrode and then was functionalized with BPA-specific aptamer via the formation of Au-S bond. The fabrication of the sensor was characterized by scanning electron microscopy and X-ray photoelectron spectroscopy. NPGF exhibited excellent electrocatalytic activity towards the redox reaction of BPA, which ensured high sensitivity of the sensor. The aptamer-captured BPA showed a pair of redox peaks around 0.35/0.28 V (vs. Ag/AgCl). The experimental parameters in terms of aptamer concentration, reaction time, pH, and temperature were optimized. The calibration plot showed a linear range from 0.1 nM to 100 nM BPA with a remarkable detection limit of 0.056±0.004 nM BPA. Particularly, the successful application of the developed sensor for the detection of BPA in human serum samples suggests its promising potential for clinical diagnosis.
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