These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Expression and characterization of LacMP, a novel fungal laccase of Moniliophthora perniciosa FA553.
    Author: Liu H, Tong C, Du B, Liang S, Lin Y.
    Journal: Biotechnol Lett; 2015 Sep; 37(9):1829-35. PubMed ID: 26093604.
    Abstract:
    OBJECTIVE: To characterize a putative laccase gene, LacMP, of Moniliophthora perniciosa FA553 that had been screened using a genome mining approach, then cloned and expressed in Pichia pastoris. RESULTS: The purified recombinant LacMP was ~57 kDa with a maximum laccase activity of 232 U/l. The optimal pH for oxidation reactions with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), syringaldazine, guaiacol and 2,6-dimethoxyphenol, were 6, 7.5, 6.5, and 6.5, respectively. The laccase activity was optimal at 60, 45, 45, and 55 °C for the four respective substrates. LacMP was stable at pH 6-8 and <30 °C. Li(+), K(+), Co(2+), Ni(2+), Mn(2+), and Mg(2+) at 1 mM had no obvious effect on its activity. CONCLUSION: Given that LacMP has optimal activity under neutral and alkaline oxidation reaction conditions, it could be a potential candidate for industrial biocatalytic applications.
    [Abstract] [Full Text] [Related] [New Search]