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  • Title: Separation of human, bovine, and porcine insulins, three very closely related proteins, by micellar electrokinetic chromatography.
    Author: Lamalle C, Roland D, Crommen J, Servais AC, Fillet M.
    Journal: Electrophoresis; 2015 Oct; 36(19):2504-6. PubMed ID: 26095856.
    Abstract:
    Human, bovine, and porcine insulins are small proteins with very closely related amino acid sequences, which makes their separation challenging. In this study, we took advantage of the high-resolution power of CE, and more particularly of micellar electrokinetic chromatography, to separate those biomolecules. Among several surfactants, perfluorooctanoic acid ammonium salt was selected. Then, using a design of experiments approach, the optimal BGE composition was found to consist of 50 mM ammonium acetate pH 9.0, 65 mM perfluorooctanoic acid ammonium salt, and 4% MeOH. The three insulins could be separated within 12 min with a satisfactory resolution. This method could be useful to detect possible counterfeit pharmaceutical formulations. Indeed, it would be easy to determine if human insulin was replaced by bovine or porcine insulin.
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