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  • Title: Complete sequence and characterization of the Silurus lanzhouensis (Siluriformes: Siluridae) mitochondrial genome.
    Author: Lian ZQ, Wu XD, Xiao W, Sai QY, Gun SB.
    Journal: Mitochondrial DNA A DNA Mapp Seq Anal; 2016 Jul; 27(4):2483-4. PubMed ID: 26171872.
    Abstract:
    The complete mitochondrial DNA (mtDNA) sequence of Silurus lanzhouensis was constructed from whole-genome Illumina sequencing data. The 16 523 bp circular genome comprises typical mtDNA components. All 13 protein-coding genes (PCGs) are initiated by an ATG except for COX1, which uses GTG. Some PCGs harbor TAG (ND2 and ND3) or an incomplete stop codon T (COX2, ND4, and CYTB), while others use TAA as their stop codon. 12S rRNA and 16S rRNA secondary structures are composed of four domains with 45 helices and six domains with 54 helices, respectively. All tRNAs are predicted to fold into the expected typical cloverleaf secondary structure except tRNA-Ser((AGN)). The largest intergenic spacer sequence was predicted to be the origin of light-strand replication. Eight conserved sequences were identified in the control region (CR). This complete S. lanzhouensis mitogenome provides useful data for further studies on molecular systematics, taxonomic status, stock evaluation, and conservation genetics.
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