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  • Title: [Continuous cell subline A4C2/9K and its application to the african swine fever virus study].
    Author: Balysheva VI, Prudnikova EY, Galnbek TV, Balyshev VM.
    Journal: Vopr Virusol; 2015; 60(2):43-7. PubMed ID: 26182658.
    Abstract:
    A new continuous cell subline A4C2/9K highly sensitive to the african swine fever virus (ASFV) was prepared. All the tested ASFV strains isolated in the Russian Federation in 2008-2013 proliferated in this cell culture exhibiting hemadsorption and accumulated at a titer of up to 6.5 Ig HAU50/cm3. The cell culture A4C2/9K can be used for ASFV isolation or determination of its infectious activity and serotype identity. The culture versions of the ASFV strain Stavropol 01/08 at passages 24 and 33 in the cell culture A4C2/9K lost their pathogenicity for pigs.
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