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  • Title: Determination of Human Hemoglobin Derivatives.
    Author: Attia AM, Ibrahim FA, Abd El-Latif NA, Aziz SW, Abdelmottaleb Moussa SA, Elalfy MS.
    Journal: Hemoglobin; 2015; 39(5):371-4. PubMed ID: 26193973.
    Abstract:
    The levels of the inactive hemoglobin (Hb) pigments [such as methemoglobin (metHb), carboxyhemoglobin (HbCO) and sulfohemoglobin (SHb)] and the active Hb [in the oxyhemoglobin (oxyHb) form] as well as the blood Hb concentration in healthy non pregnant female volunteers were determined using a newly developed multi-component spectrophotometric method. The results of this method revealed values of SHb% in the range (0.0727-0.370%), metHb% (0.43-1.0%), HbCO% (0.4-1.52%) and oxyHb% (97.06-98.62%). Furthermore, the results of this method revealed values of blood Hb concentration in the range (12.608-15.777 g/dL). The method is highly sensitive, accurate and reproducible.
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