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  • Title: [Comparative studies of the suitability of different culture media for the isolation of Mycoplasma californicum from milk samples].
    Author: Pfützner H, Höfer M.
    Journal: Arch Exp Veterinarmed; 1989; 43(5):699-704. PubMed ID: 2619463.
    Abstract:
    Mycoplasma (M.) californicum und M. bovigenitalium mastitis had occurred in four herds. 240 milk samples of 120 cows were tested for M. californicum, using in parallel nine different culturing substrates and test methods. Included were 120 initial and 120 final milk samples. Inoculation of milk to medium-I broth containing V-factor yeast extract, incubation at 37 degrees C for 5 days, subsequent spreading onto cattle blood agar with staphylococcal nutrix, followed by 5 days of incubation proved to be superior to all other culturing substrates and methods tested and resulted in positive detection of M. californicum in 69 milk samples. M. californicum was identified in 70 milk samples by combination of all 9 test variants. No significant difference was found to exist with regard to isolation results between initial and final milking samples.
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