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  • Title: SLAP: Small Labeling Pair for Single-Molecule Super-Resolution Imaging.
    Author: Wieneke R, Raulf A, Kollmannsperger A, Heilemann M, Tampé R.
    Journal: Angew Chem Int Ed Engl; 2015 Aug 24; 54(35):10216-9. PubMed ID: 26201868.
    Abstract:
    Protein labeling with synthetic fluorescent probes is a key technology in chemical biology and biomedical research. A sensitive and efficient modular labeling approach (SLAP) was developed on the basis of a synthetic small-molecule recognition unit (Ni-trisNTA) and the genetically encoded minimal protein His6-10 -tag. High-density protein tracing by SLAP was demonstrated. This technique allows super-resolution fluorescence imaging and fulfills the necessary sampling criteria for single-molecule localization-based imaging techniques. It avoids masking by large probes, for example, antibodies, and supplies sensitive, precise, and robust size analysis of protein clusters (nanodomains).
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