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  • Title: Highly charged cellulose-based nanocrystals as flocculants for harvesting Chlorella vulgaris.
    Author: Vandamme D, Eyley S, Van den Mooter G, Muylaert K, Thielemans W.
    Journal: Bioresour Technol; 2015 Oct; 194():270-5. PubMed ID: 26210139.
    Abstract:
    This study presents a novel flocculant for harvesting Chlorella vulgaris as model species for freshwater microalgae based on cellulose nanocrystals (CNCs), thus synthesized from a renewable and biodegradable resource. Cationic pyridinium groups were grafted onto CNCs by two separate one-pot simultaneous esterification and nucleophilic substitution reactions. Both types of modified CNCs were positively charged in the pH range 4-11. Both reactions yielded CNCs with a high degree of substitution (up to 0.38). A maximum flocculation efficiency of 100% was achieved at a dosage of 0.1 g g(-1) biomass. In contrast to conventional polymer flocculants, cationic CNCs were relatively insensitive to inhibition of flocculation by algal organic matter. The present results highlight the potential of these new type of nanocellulose-based flocculants for microalgae harvesting.
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