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  • Title: [Molecularly imprinted polymers in electro analysis of proteins].
    Author: Shumyantseva VV, Bulko TV, Baychorov IKh, Archakov AI.
    Journal: Biomed Khim; 2015; 61(3):325-31. PubMed ID: 26215409.
    Abstract:
    In the review the main approaches to creation of recognition materials capable of competing with biological specific receptors, (polymeric analogs of antibodies or molecularly imprinted polymers, MIP) for the electro analysis of functionally significant proteins such as a myoglobin, troponin T, albumin, human ferritin, calmodulin are considered. The main types of monomers for MIP fabrication, and methods for MIP/protein interactions, such as a surface plasmon resonance (SPR), nanogravimetry with use of the quartz crystal resonator (QCM), spectral and electrochemical methods are discussed. Experimental data on electrochemical registration of a myoglobin using MIP/electrode are presented. For a development of electrochemical sensor systems based on MIPs, o-phenylenediamine (1,2-diaminobenzene was used as a monomer. It was shown that the imprinting factor Imax(MIP)/Imax(NIP), calculated as a myoglobin signal ratio when embedding in MIP to a myoglobin signal when embedding in the polymer received without molecular template (NIP) corresponds 2-4. Rassmotreny osnovnye podkhody k sozdaniiu vysokoaffinnykh polimernykh analogov antitel (polimerov s molekuliarnymi otpechatkami, MIP) dlia élektroanaliza funktsional'no znachimykh belkovykh molekul, takikh kak mioglobin, troponin T, al'bumin, ferritin, lizotsim, kal'modulin. Proanalizirovany osnovnye tipy monomerov dlia polucheniia polimerov s molekuliarnymi otpechatkami, a takzhe metody analiza vzaimodeĭstviĭ MIP-belok, takie kak plazmonnyĭ poverkhnostnyĭ rezonans (SPR), nanogravimetriia s ispol'zovaniem kvartsevogo rezonatora (QCM), spektral'nye i élektrokhimicheskie metody. Privedeny dannye po élektrokhimicheskoĭ registratsii mioglobina na élektrodakh, modifitsirovannykh MIP na osnove o-fenilendiamina. Pokazano, chto faktor imprintinga, rasschitannyĭ kak sootnoshenie signala mioglobina pri vstraivanii v molekuliarno imprintirovannyĭ polimer k signalu mioglobina pri vstraivanii v polimer, poluchennyĭ bez molekuliarnykh otpechatkov, sostavliaet 2-4.
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