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  • Title: Comparative study of methods of detection of hepatitis 'B' surface antigen (HBsAg).
    Author: Parab VV, Talawadekar NN, Kale VV, Kamat SA.
    Journal: J Postgrad Med; 1989 Apr; 35(2):83-5. PubMed ID: 2621667.
    Abstract:
    The serum samples were collected from 52 patients of acute viral hepatitis and 235 hospital staff from Kasturba Hospital for Infectious Diseases. HBsAg was detected in their sera by counter-immuno-electrophoresis (CIEP), reverse passive hemogglutination (RPHA) and by micro-enzyme-linked-immunosorbent assay (ELISA) technique. Among the patients, HBsAg was detected in 12 cases (23%) by CIEP, in 18 cases (34%) by RPHA and in 23 patients (45%) by ELISA. In the hospital staff, HBsAg was detected in 4 samples (1.7%) by CIEP, in 8 samples (3.5%) by RPHA and in 32 samples (13.5%) by ELISA. Thus ELISA was found to be the most sensitive technique in detecting HBsAg.
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