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  • Title: Chemical and Biological Investigation of Olive Mill Waste Water - OMWW Secoiridoid Lactones.
    Author: Vougogiannopoulou K, Angelopoulou MT, Pratsinis H, Grougnet R, Halabalaki M, Kletsas D, Deguin B, Skaltsounis LA.
    Journal: Planta Med; 2015 Aug; 81(12-13):1205-12. PubMed ID: 26218340.
    Abstract:
    Olive mill waste water is the major byproduct of the olive oil industry containing a range of compounds related to Olea europaea and olive oil constituents. Olive mill waste water comprises an important environmental problem in olive oil producing countries, but it is also a valuable material for the isolation of high added value compounds. In this study, an attempt to investigate the secoiridoid content of olive mill waste water is described with the aid of ultrahigh-performance liquid chromatography-electrospray ionization (±)-high-resolution mass spectrometry and centrifugal partition chromatography methods. In total, seven secoiridoid lactones were isolated, four of which are new natural products. This is the first time that a conjugate of hydroxytyrosol and a secoiridoid lactone has been isolated from olive mill waste water and structurally characterized. Furthermore, the range of isolated compounds allowed for the proposal of a hypothesis for the biotransformation of olive secoiridoids during the production of olive mill waste water. Finally, the ability of the representative compounds to reduce the intracellular reactive oxygen species was assessed with the dichlorofluorescein assay in conjunction with the known antioxidant agent hydroxytyrosol.
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