These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Effect of jiaweiwumel decoction on regulatory T cells and interleukin-10 in a rat model of ulcerative colitis.
    Author: Tao M, Wang X, Wang A, Gao N, Pan J, Liu X, Wang X.
    Journal: J Tradit Chin Med; 2015 Jun; 35(3):312-5. PubMed ID: 26237836.
    Abstract:
    OBJECTIVE: To investigate the effect of a decoction made from the Traditional Chinese Medicine wumei pill, on regulatory T cells and interleukin-10 (IL-10) in a rat model of ulcerative colitis induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS). METHODS: Rat ulcerative colitis was induced with TNBS. All modeled rats were randomly divided into six groups: normal control group; model group; sulfasalazine suppositories treatment group; and high, moderate, and low dosage of jiaweiwumei decoction groups (12 rats each). Colon injury index was evaluated after 14 days. After peripheral blood lymphocyte separation, CD4+ T cells and CD4+/CD25+ T cell percentage was detected by flow cytometry. The content of IL-10 in serum and intestinal mucosa tissue was detected by sandwich enzyme-linked immunosorbent assay. RESULTS: Colon injury indices in the decoction groups were effectively reduced, compared with the model group (P < 0.05). Compared with that of the control group, the CD4+/CD25+ to CD4+ T lymphocyte ratio of the model group was significantly lower, while the decoction treatment improved the CD4 +/CD25 + to CD4 + T lymphocyte ratio (P < 0.05). The serum and mucosal IL-10 content of the model group was significantly lower (P < 0.05) than that in the control group, while the decoction group had significantly higher serum and intestinal mucosal IL-10 content than that in the model group (P < 0.05). The regulatory T cell content was negatively correlated with the colonic injury index (r = 0.68, P < 0.05), and positively correlated with the content of serum IL-10 (r= 0.87, P < 0.05) and intestinal mucosal IL-10 (r= 0.79, P < 0.05). CONCLUSION: Jiaweiwumei decoction had significant effects on regulatory T cells and IL-10 in rats with TNBS-induced ulcerative colitis.
    [Abstract] [Full Text] [Related] [New Search]