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  • Title: Maribacter confluentis sp. nov., isolated from the junction between the ocean and a freshwater spring.
    Author: Park S, Jung YT, Park JM, Won SM, Yoon JH.
    Journal: Int J Syst Evol Microbiol; 2015 Sep; 65(9):3079-3085. PubMed ID: 26297027.
    Abstract:
    A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated, non-gliding and rod-shaped bacterial strain, SSK2-2(T), was isolated from the place where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain SSK2-2(T) grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0-3.0% (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain SSK2-2(T) fell within the clade comprising the type strains of species of the genus Maribacter, joining the type strain of Maribacter sedimenticola with which it shared 99.4% similarity. Sequence similarities to the type strains of other Maribacter species were 94.6-98.2%. Strain SSK2-2(T) contained MK-6 as the predominant menaquinone and iso-C17 : 0 3-OH, iso-C15 : 0, iso-C15 : 1 G, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 9 (iso-C17 : 1ω9c and/or 10-methyl C16 : 0) as the major fatty acids. The major polar lipids of strain SSK2-2(T) were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain SSK2-2(T) was 38.2 mol% and mean levels of DNA-DNA relatedness with the type strains of four phylogenetically related species of the genus Maribacter were 11-24%. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain SSK2-2(T) is separate from other Maribacter species. On the basis of the data presented, strain SSK2-2(T) is considered to represent a novel species of the genus Maribacter, for which the name Maribacter confluentis sp. nov. is proposed. The type strain is SSK2-2(T) ( = KCTC 42604(T) = CECT 8869(T)).
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