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  • Title: Biotransformation of lysine into cadaverine using barium alginate-immobilized Escherichia coli overexpressing CadA.
    Author: Bhatia SK, Kim YH, Kim HJ, Seo HM, Kim JH, Song HS, Sathiyanarayanan G, Park SH, Park K, Yang YH.
    Journal: Bioprocess Biosyst Eng; 2015 Dec; 38(12):2315-22. PubMed ID: 26314400.
    Abstract:
    In this study, Escherichia coli cells overexpressing lysine decarboxylase (CadA) were used for cadaverine production. Barium alginate was selected as a matrix for immobilization of E. coli YH91. Free cells and immobilized cells (IC) were characterized for their physiochemical properties, and the optimum pH and temperature were determined as 6 and 37 °C, respectively. Immobilized cells were three times more thermally stable compared to free cells at the optimum temperature and had a half-life (t 1/2) of 131 h. The free cells lost most of lysine decarboxylase activity after nine cycles, but in contrast immobilized cells retained 56% of their residual activity even after the 18th cycle. The immobilized cells gave a maximum production of cadaverine (75.8 g/L) with 84% conversion.
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