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  • Title: PCR for the Specific Detection of an Escherichia coli O157:H7 Laboratory Control Strain.
    Author: Knowles M, Lambert D, Huszczynski G, Gauthier M, Blais BW.
    Journal: J Food Prot; 2015 Sep; 78(9):1738-44. PubMed ID: 26319729.
    Abstract:
    Control strains of bacterial pathogens such as Escherichia coli O157:H7 are commonly processed in parallel with test samples in food microbiology laboratories as a quality control measure to assure the satisfactory performance of materials used in the analytical procedure. Before positive findings can be reported for risk management purposes, analysts must have a means of verifying that pathogenic bacteria (e.g., E. coli O157:H7) recovered from test samples are not due to inadvertent contamination with the control strain routinely handled in the laboratory environment. Here, we report on the application of an in-house bioinformatic pipeline for the identification of unique genomic signature sequences in the development of specific oligonucleotide primers enabling the identification of a common positive control strain, E. coli O157:H7 (ATCC 35150), using a simple PCR procedure.
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