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  • Title: Enhancing the efficiency of 5-aminolevulinic acid-mediated photodynamic therapy using 5-fluorouracil on human melanoma cells.
    Author: Tahmasebi H, Khoshgard K, Sazgarnia A, Mostafaie A, Eivazi MT.
    Journal: Photodiagnosis Photodyn Ther; 2016 Mar; 13():297-302. PubMed ID: 26321747.
    Abstract:
    BACKGROUND: 5-Aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) is an effective and noninvasive modality for treatment of several types of non-melanoma skin cancers. This in-vitro study attempted to know whether the killing effect of ALA-PDT on the human melanoma cells (Mel-Rm cell line) could be increased by the presence of 5-fluorouracil (5-FU). METHODS: To evaluate the effect of ALA-PDT in combination with 5-FU on viability of human melanoma Mel-Rm cells, the cells incubated with 5-ALA and 5-FU for 3h in nontoxic concentrations, and subsequently illuminated with a 630 nm light-emitting diode array. The cells viability and cytotoxicity determined by mitochondrial activity and lactate dehydrogenase assays. RESULTS: Combination of ALA-PDT and 5-FU (FU-ALA-PDT) showed a considerable growth inhibition according to the results of MTT assay compared to ALA-PDT. The results of LDH assay also showed a cytotoxicity effect in ALA-PDT; however, the FU-ALA-PDT showed no significantly enhancement in cytotoxicity compared to ALA-PDT using LDH assay. CONCLUSION: The Mel-Rm cells incubation with 5-FU before PDT enhances the efficiency of 5-Aminolevulinic acid-mediated photodynamic therapy.
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