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  • Title: Effect of magnesium ion on crt gene expression in improving carotenoid yield of Rhodobacter sphaeroides.
    Author: Liu S, Li X, Zhang G, Zhang J.
    Journal: Arch Microbiol; 2015 Nov; 197(9):1101-8. PubMed ID: 26371061.
    Abstract:
    This study aimed at increasing carotenoid yield of Rhodobacter sphaeroides in wastewater treatment by adding magnesium ion (Mg(2+)). Results showed that Mg(2+) could improve R. sphaeroides biomass and carotenoid yield effectively. The highest carotenoid yield of 4.83 ± 0.14 mg/g biomass and biomass production of 3900 ± 180 mg/L were achieved at optimal Mg(2+) concentration of 15 mmol/L. Mechanism analysis revealed that Mg(2+) could promote carotenoid production by regulating the expressions of crt genes. Up-regulation of crtBDA genes improved carotenoid biosynthesis of R. sphaeroides.
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