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  • Title: [Value of the LEN (LISS-enzyme) technic in detection of alloantibodies to erythrocytes].
    Author: Walewska I, Kuśnierz-Alejska G, Michalewska B.
    Journal: Acta Haematol Pol; 1989; 20(2):214-22. PubMed ID: 2638541.
    Abstract:
    The reported study was done for assessment of the value of the enzymatic technique LSE/LISS Spin Enzyme proposed by Odell et al. using a solution of NaCl of low ionic strength (LISS) for detection of incomplete alloantibodies to erythrocytes, especially those from the Rh system. The results of the study demonstrated that the LSE test, called by the authors LEN (LISS-Enzyme) is a sensitive and specific method comparable in its effectiveness to the two-step papain test (2-Pap) used widely in this country. In routine studies the LEN technique is simple, easy and rapid. Washing of papain treated erythrocytes is not necessary. The time from enzyme addition to erythrocytes to result reading is about 15 minutes, that is it is four times shorter than the time in the 2-Pap technique (60 minutes). A significant was the preparation of one type of erythrocyte suspension in LISS solution for two tests: enzymatic and antiglobulin, carried out in parallel. For these reasons the LEN test may be recommended in place of 2-Pap for detection and identification of antibodies. It is also a practical methods for matching test in place of the one-step papain test. The LEN test should be widely introduced in laboratories of transfusiological immunology in this country. Attention is called ti various technical details necessary for obtaining of unequivocal and reproducible results.
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