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  • Title: Detection of common dermatophytes in clinical specimens using a simple quantitative real-time TaqMan polymerase chain reaction assay.
    Author: Ohst T, Kupsch C, Gräser Y.
    Journal: Br J Dermatol; 2016 Mar; 174(3):602-9. PubMed ID: 26406273.
    Abstract:
    BACKGROUND: Dermatophytes are common fungal pathogens causing mostly superficial infections in humans with a high prevalence worldwide. Traditional detection techniques are time-consuming and insensitive, whereas molecular detection methods have proved to be much more rapid and sensitive. OBJECTIVES: To develop a modular singleplex quantitative real-time polymerase chain reaction (qRT-PCR) assay for the detection of the most common dermatophytes in clinical specimens. METHODS: The qRT-PCR assay is based on single-tube reactions with TaqMan probes. We validated the test with 311 clinical samples of human and animal origin submitted for routine diagnosis and compared the qRT-PCR results with microscopy and culture. RESULTS: qRT-PCR proved to be significantly more sensitive than microscopy and culture, with 21·2% more positive samples. Among the 201 dermatophytes identified 152 were Trichophyton rubrum (75·6%) and 34 were Trichophyton interdigitale (16·9%). Only 15 samples were determined as less common dermatophytes (Microsporum canis, Epidermophyton floccosum, Trichophyton verrucosum and Arthroderma benhamiae). In the present study, pathogen identification was achieved for 95·2% of all samples (including negatives) by applying only three detection tests (pan-dermatophyte, T. rubrum and T. interdigitale). CONCLUSIONS: The qRT-PCR assay developed in this study allows the specific and sensitive detection of relevant dermatophytes at low cost in a short time.
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