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  • Title: [Pre-analytical stability before centrifugation of 7 biochemical analytes in whole blood].
    Author: Perrier-Cornet A, Moineau MP, Narbonne V, Plee-Gautier E, Le Saos F, Carre JL.
    Journal: Ann Biol Clin (Paris); 2015; 73(4):454-60. PubMed ID: 26411912.
    Abstract:
    The pre-analytical stability of 7 biochemical parameters (parathyroid hormone -PTH-, vitamins A, C E and D, 1,25-dihydroxyvitamin D and insulin) at +4 °C, was studied on whole blood samples before centrifugation. The impact of freezing at -20°C was also analyzed/performed for PTH and vitamin D. The differences in the results of assays for whole blood samples, being kept for different times between sampling time and analysis, from 9 healthy adults, were compaired by using a Student t test. The 7 analytes investigated remained stable up to 4 hours at +4°C in whole blood. This study showed that it is possible to accept uncentrifuged whole blood specimens kept at +4°C before analysis. PTH is affected by freezing whereas vitamin D is not.
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