These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Solvent extraction and high performance liquid chromatography with electrochemical detection for determination of plasma catecholamines].
    Author: Zhang L, Zhao WK.
    Journal: Zhongguo Yao Li Xue Bao; 1989 Nov; 10(6):572-5. PubMed ID: 2641860.
    Abstract:
    This paper describes a technique for selectively extracting plasma catecholamines prior to quantification by HPLC-EC. The extraction system was a two-stage process. The first stage involve the complex formation between diphenylbrorate and catechol (diol) groups in alkaline medium. The second stage was a liquid-liquid extraction. The complex combined with tetraoctylammonium bromide to form an ion-pair formation into organic solvent. The catecholamines in turn was extracted with acid. This technique provided a very specific extraction procedure which resulted in chromatograms with few interfering compounds and gave absolute recoveries (100-103%) of norepinephrine, epinephrine and dopamine. Meanwhile, the plasma catecholamines were concentrated and the detective sensitivity was increased. A good linear relationship was found between the concentrations and ratio of peak heights of the catecholamines from 0.125-2 ng. The correlation coefficients ranged from 0.998-0.999. The coefficients of variation of the intra- and inter-assay were within 3 and 6% respectively. The results show that the procedure is very simple and fast. The method is valuable not only for clinical diagnosis but also for laboratory research.
    [Abstract] [Full Text] [Related] [New Search]