These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Expression of the pneumolysin gene in Escherichia coli: rapid purification and biological properties.
    Author: Mitchell TJ, Walker JA, Saunders FK, Andrew PW, Boulnois GJ.
    Journal: Biochim Biophys Acta; 1989 Jan 23; 1007(1):67-72. PubMed ID: 2642385.
    Abstract:
    The gene for pneumolysin, the thiol-activated toxin from Streptococcus pneumoniae, has been expressed in Escherichia coli. The recombinant protein has been purified using a rapid, high yield, purification procedure and has been shown to be identical with respect to N-terminal amino-acid sequence, specific activity, effect on human polymorphonuclear phagocytes and effect on human complement to the native toxin purified from the pneumococcus. This provides a large enough source of material to begin investigation of pneumolysin as a candidate for inclusion in a pneumococcal vaccine.
    [Abstract] [Full Text] [Related] [New Search]