These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Humoral regulation of eosinophilopoiesis in vitro: analysis of the targets of interleukin-3, granulocyte/macrophage colony-stimulating factor (GM-CSF), and interleukin-5.
    Author: Sonoda Y, Arai N, Ogawa M.
    Journal: Leukemia; 1989 Jan; 3(1):14-8. PubMed ID: 2642572.
    Abstract:
    In order to delineate the humoral regulation of eosinophil production, we studied the effects of interleukin-3 (IL-3), granulocyte/macrophage colony-stimulating factor (GM-CSF), and interleukin-5 (IL-5), and their combinations on eosinophil colony formation in clonal cell culture. We plated 1,000 bone marrow null cells per dish and in some experiments used polyclonal anti-gibbon IL-3 sera and anti-human GM-CSF. IL-3 or GM-CSF independently from each other supported eosinophil colony formation. Although IL-5 supported formation of small eosinophil colonies, the number of colonies were significantly smaller than that supported by GM-CSF or IL-3. Cytological examination of the constituent cells revealed that some of the apparent eosinophil colonies supported by IL-3 and GM-CSF were mixed colonies containing eosinophils and one or more other lineages. In addition, the majority of the eosinophils seen in cultures with IL-3 and/or GM-CSF proved to be early eosinophil precursors including eosinophilic promyelocytes, myelocytes, and meta-myelocytes. IL-5-supported eosinophil colonies were pure eosinophil colonies and contained mostly maturer eosinophils such as band and segmented forms. These observations indicated that the developmental stages of the targets of IL-3 and GM-CSF are earlier than those of IL-5 and that the primary function of IL-5 is to support terminal maturation of eosinophils.
    [Abstract] [Full Text] [Related] [New Search]