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  • Title: Assembly of Customized TAL Effectors Through Advanced ULtiMATE System.
    Author: Yang J, Guo S, Yuan P, Wei W.
    Journal: Methods Mol Biol; 2016; 1338():49-60. PubMed ID: 26443213.
    Abstract:
    Transcription activator-like effectors (TALEs) have been widely applied in gene targeting. Here we describe an advanced ULtiMATE (USER-based Ligation-Mediated Assembly of TAL Effector) system that utilizes USER fusion technique and archive of 512 tetramer templates to achieve highly efficient construction of TALEs, which takes only half a day to accomplish the assembly of any given TALE construct. This system is also suitable for large-scale assembly of TALENs and any other TALE-based constructions.
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