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  • Title: A competitive enzyme-linked immunosorbent assay for detecting Escherichia coli heat-stable enterotoxin and its application to clinical isolates.
    Author: Cryan B.
    Journal: J Infect; 1989 Jan; 18(1):59-66. PubMed ID: 2644360.
    Abstract:
    Enterotoxigenic Escherichia coli (ETEC) are well recognised enteric pathogens. The epidemiology of heat-stable enterotoxin (STa) producing strains has not been established due mainly to difficulties encountered in performing bioassays on a large scale in baby mice. This study describes a competitive enzyme-linked immunosorbent assay (ELISA) for detecting STa and its application to clinical isolates. Compared to the bioassay, the ELISA had a specificity of 93.7% and a sensitivity of 90.9%. It detected as little as 1 microgram/l STa. Of 720 E. coli isolates from children with diarrhoea, 69 (9.6%) were positive for STa by ELISA.
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