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  • Title: Agglutination Assays of the Plasmodium falciparum-Infected Erythrocyte.
    Author: Tan J, Bull PC.
    Journal: Methods Mol Biol; 2015; 1325():115-29. PubMed ID: 26450384.
    Abstract:
    The agglutination assay is used to determine the ability of antibodies to recognize parasite variant antigens on the surface of Plasmodium falciparum-infected erythrocytes. In this technique, infected erythrocytes are selectively labelled with a DNA-binding fluorescent dye and mixed with antibodies of interest to allow antibody-surface antigen binding. Recognition of surface antigens by the antibodies can result in the formation of agglutinates containing multiple parasite-infected erythrocytes. These can be viewed and quantified using a fluorescence microscope.
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