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  • Title: In-capillary self-assembly and proteolytic cleavage of polyhistidine peptide capped quantum dots.
    Author: Wang J, Li J, Li J, Liu F, Zhou X, Yao Y, Wang C, Qiu L, Jiang P.
    Journal: Anal Chim Acta; 2015 Oct 01; 895():112-7. PubMed ID: 26454466.
    Abstract:
    A new method using fluorescence coupled capillary electrophoresis (CE-FL) for monitoring self-assembly and proteolytic cleavage of hexahistidine peptide capped quantum dots (QDs) inside a capillary has been developed in this report. QDs and the ATTO 590-labeled hexahistidine peptide (H6-ATTO) were injected into a capillary, sequentially. Their self-assembly inside the capillary was driven by a metal-affinity force which yielded a new fluorescence signal due to Förster resonance energy transfer (FRET). The highly efficient separation of fluorescent complexes and the FRET process were analyzed using CE-FL. The self-assembly of QDs and biomolecules was found to effectively take place inside the capillary. The kinetics of the assembly was monitored by CE-FL, and the approach was extended to the study of proteolytic cleavage of surface conjugated peptides. Being the first in-depth analysis of in-capillary nanoparticle-biomolecule assembly, the novel approach reported here provides inspiration to the development of QD-based FRET probes for biomedical applications.
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