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  • Title: A simple and sensitive fluorimetric aptasensor for the ultrasensitive detection of arsenic(III) based on cysteamine stabilized CdTe/ZnS quantum dots aggregation.
    Author: Ensafi AA, Kazemifard N, Rezaei B.
    Journal: Biosens Bioelectron; 2016 Mar 15; 77():499-504. PubMed ID: 26457735.
    Abstract:
    A new approach for developing a fluorimetric aptasensor has been described and applied for determination of a highly toxic cation, As(III). In this method an aptamer was used to aggregate cationic cysteamine-stabilized CdTe/ZnS core/shell quantum dots, as a result fluorescence quenching was accrued. In the presence of As(III), the aptamer and As(III) make a complex, which prevents aggregation of the quantum dots. Thus, the fluorescence intensity of the quantum dots was enhanced upon the de-aggregation, which depends on the concentration of As(III). The fluorimetric assay has a very low detection limit of 1.3 pmolL(-1) As(III) with a dynamic range of 1.0 × 10(-11) to 1.0 × 10(-6) molL(-1). The interference effect of a wide variety of cations and anions was investigated, and the obtained results confirm high selectivity of the aptasensor for As(III) detection. The present assay was successfully applied for the determination of As(III) in several water samples.
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