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  • Title: PpNrg1 is a transcriptional repressor for glucose and glycerol repression of AOX1 promoter in methylotrophic yeast Pichia pastoris.
    Author: Wang X, Cai M, Shi L, Wang Q, Zhu J, Wang J, Zhou M, Zhou X, Zhang Y.
    Journal: Biotechnol Lett; 2016 Feb; 38(2):291-8. PubMed ID: 26463371.
    Abstract:
    OBJECTIVE: The regulator in glycerol repression of Pichia pastoris AOX1 promoter (P AOX1 ) is still unclear. RESULTS: A Cys2His2 zinc finger transcriptional repressor PpNrg1 localized to nucleus and participated in the repression of P AOX1 in P. pastoris in glucose and glycerol. Quantitative real-time PCR revealed that PpNrg1 repressed expression of numerous genes involved in methanol utilization and peroxisome biogenesis in 0.02 % glucose and 1 % (v/v) glycerol. Electrophoretic mobility shift assay and DNase I footprinting assay revealed that PpNrg1 bound to five sites of P AOX1 , including two binding sites of PpMxr1, which is an indispensable activator of P AOX1 in P. pastoris. CONCLUSION: Transcriptional repressor PpNrg1 suppresses P AOX1 in glucose and glycerol by directly binding to five sites of P AOX1 , including two binding sites of transcriptional activator PpMxr1.
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