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  • Title: Studying the Translatome with Polysome Profiling.
    Author: Zuccotti P, Modelska A.
    Journal: Methods Mol Biol; 2016; 1358():59-69. PubMed ID: 26463377.
    Abstract:
    Polysome fractionation by sucrose density gradient centrifugation followed by analysis of RNA and protein is a technique that allows to understand the changes in translation of individual mRNAs as well as genome-wide effects on the translatome. Here, we describe the polysome profiling technique and RNA as well as protein isolation procedures from sucrose fractions.
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