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  • Title: Accuracy of Conventional PCR Targeting the 16S rRNA Gene with the Ot-16sRF1 and Ot-16sRR1 Primers for Diagnosis of Scrub Typhus: a Case-Control Study.
    Author: Kim CM, Cho MK, Kim DM, Yun NR, Kim SW, Jang SJ, Ahn YJ, Lim D.
    Journal: J Clin Microbiol; 2016 Jan; 54(1):178-9. PubMed ID: 26491185.
    Abstract:
    We retrospectively evaluated the accuracy of conventional PCR targeting the 16S rRNA gene (16S C-PCR) using the Ot-16sRF1/Ot-16sRR1 primers for diagnosing scrub typhus. The diagnosis of Orientia tsutsugamushi infection by 16S C-PCR presented an increased sensitivity of 87.0% and specificity of 100% compared with those obtained with other targets and is thus a simple and clinically useful method with good diagnostic accuracy.
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