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Title: Use of gene replacement to construct Escherichia coli strains carrying mutations in two genes required for stability of multicopy plasmids. Author: Flinn H, Burke M, Stirling CJ, Sherratt DJ. Journal: J Bacteriol; 1989 Apr; 171(4):2241-3. PubMed ID: 2649493. Abstract: Escherichia coli mutants completely defective in ColE1 cer-mediated site-specific recombination have been mapped to two genes, xerA and xerB. In this study, xerA xerB double mutants were constructed by gene replacement with a lambda dv plasmid and were shown to be both viable and defective in ColE1 site-specific recombination.[Abstract] [Full Text] [Related] [New Search]