These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Proximity hybridization-regulated electrochemical stripping of silver nanoparticles via nanogold induced deposition for immunoassay.
    Author: Li J, Wu J, Cui L, Liu M, Yan F, Ju H.
    Journal: Analyst; 2016 Jan 07; 141(1):131-6. PubMed ID: 26523811.
    Abstract:
    A simple and disposable electrochemical immunosensor was developed for sensitive and selective detection of a protein biomarker via target-induced proximity hybridization and electrochemical stripping analysis of silver nanoparticles (AgNPs). The immunosensor was prepared by assembling single-stranded DNA modified gold nanoparticles (ssDNA@AuNPs) on a graphene oxide modified disposable screen-printed carbon electrode (SPCE). In the presence of a target protein and two DNA-labeled antibodies, the proximate complex formed in homogeneous solution could hybridize with the assembled DNA to take away AuNPs, which decreased AuNP-catalyzed deposition of AgNPs on the immunosensor surface, and thus the anodic stripping signal. The proposed method avoided the interference of dissolved oxygen. Using the carcinoembryonic antigen (CEA) as a model analyte, this method showed a linear range of four magnitude orders with a detection limit down to 3.9 pg mL(-1). The electrochemical immunosensor possessed preparation convenience, good stability and high sensitivity, and could be extended to sensitive biosensing of other analytes, showing potential application in point-of-care testing.
    [Abstract] [Full Text] [Related] [New Search]