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  • Title: Synthesis of radiolabeled acetyl-coenzyme A from sodium acetate.
    Author: Clough RC, Barnum SR, Jaworski JG.
    Journal: Anal Biochem; 1989 Jan; 176(1):82-4. PubMed ID: 2653102.
    Abstract:
    The synthesis of high specific radioactivity [14C]-acetyl-Coenzyme A from [14C]sodium acetate, 2,6-dichlorobenzoic acid, 1,1'-carbonyldiimidazole, and CoA is reported. Starting with 1 mumol of [14C]sodium acetate, this method yields pure [14C]acetyl-CoA in yields approaching 40%. Chromatography on a reversed-phase ODS column was used to separate acetyl-CoA from Coenzyme A and side products. The acetylating agent is apparently a reaction intermediate, acetylimidazole.
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