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  • Title: Nucleotide sequence of the F plasmid transfer gene, traH: identification of a new gene and a promoter within the transfer operon.
    Author: Ham LM, Firth N, Skurray R.
    Journal: Gene; 1989 Jan 30; 75(1):157-65. PubMed ID: 2656408.
    Abstract:
    The nucleotide sequence of the F plasmid transfer gene traH, which is involved in F-pilus assembly in Escherichia coli K-12, has been determined. From the sequence data, it would appear that traH encodes a 38,897-dalton precursor polypeptide which is processed to give a periplasmic protein. Furthermore, a new gene, trbF, has been located immediately upstream of traH and shown to be expressed by means of a translational fusion to lacZ. Using galK fusion and S1 nuclease protection studies, a weak traJ-dependent promoter, P trbF, has been mapped upstream and adjacent to trbF. Transcription of trbF and traH from P trbF may well serve to complement transcription from the major tra operon promoter PY located some 16 kb upstream of these genes.
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