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  • Title: A cold-adapted and glucose-stimulated type II α-glucosidase from a deep-sea bacterium Pseudoalteromonas sp. K8.
    Author: Li W, Xue Y, Li J, Yuan J, Wang X, Fang W, Fang Z, Xiao Y.
    Journal: Biotechnol Lett; 2016 Feb; 38(2):345-9. PubMed ID: 26564409.
    Abstract:
    OBJECTIVES: To express and characterize a putative α-glucosidase, Pagl, from Pseudoalteromonas sp. K8 obtained via genome mining approach. RESULTS: Pagl was expressed and purified to homogeneity, with a molecular mass of 60 kDa. It was optimally active at pH 8.5 and 30 °C, and showed cold-adapted activity. Pagl exhibited specific activity towards substrates with α-1,4-linkage, with the highest specific activity of 19.4 U/mg for maltose, followed by pNPαG and maltodextrins, suggesting that Pagl belongs to the type II α-glucosidase. Interestingly, the activity of Pagl is significantly enhanced (2.7 times) in the presence of 200 mM glucose. CONCLUSION: The unique catalytic properties of Pagl make it an attractive candidate for several industrial applications.
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