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  • Title: N myristylation of the human immunodeficiency virus type 1 gag polyprotein precursor in Saccharomyces cerevisiae.
    Author: Bathurst IC, Chester N, Gibson HL, Dennis AF, Steimer KS, Barr PJ.
    Journal: J Virol; 1989 Jul; 63(7):3176-9. PubMed ID: 2657103.
    Abstract:
    A semisynthetic gene precisely encoding the 502 amino acids of the human immunodeficiency virus type 1 gag precursor (Pr53gag) was expressed in the yeast Saccharomyces cerevisiae. Amino acid sequence analysis of the recombinant Pr53gag showed that the amino terminus was fully blocked. Labeling of Pr53gag with [3H]myristic acid demonstrated that, as with Pr53gag isolated from virus-infected cells, the yeast-derived protein was demethionylated and N myristylated on glycine, the second amino acid residue.
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