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  • Title: EFFECT OF SEMEN EXTENDER SUPPLEMENTATION WITH TREHALOSE，VITAMIN C AND E ON POST-THAW MIN PIG SPERM QUALITIES.
    Author: Ma H, Liu D, Wang W, Wang L, Fu B, Li Z, He X.
    Journal: Cryo Letters; 2015; 36(5):308-12. PubMed ID: 26574677.
    Abstract:
    BACKGROUND: Cryopreservation of min pig sperm offers an effective method to protect this valuable genetic resource. OBJECTIVE: To determine whether the extender supplementation with some antioxidants is able to improve the quality of post-thawed sperm of min pig. MATERIALS AND METHODS: Ejaculated min pig sperm frozen in lactose-egg yolk (LEY) extender supplemented with trehalose, vitamin C and/or water-soluble vitamin E were thawed, and the quality of sperm samples were evaluated. RESULTS: The quality of post-thawed sperm samples was higher in the groups with supplementation than that of the control group (P < 0.05). Furthermore, the addition of 100 mM trehalose and 25 mM water-soluble vitamin E to the freezing extender strongly preserved the quality of the sperm (P < 0.05). CONCLUSION: Trehalose and water-soluble vitamin E in the freezing extender effectively improved post-thaw qualities of min pig sperm.
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