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  • Title: Identification and characterization of a novel chitinase with antifungal activity from 'Baozhu' pear (Pyrus ussuriensis Maxim.).
    Author: Han P, Yang C, Liang X, Li L.
    Journal: Food Chem; 2016 Apr 01; 196():808-14. PubMed ID: 26593558.
    Abstract:
    A novel chitinase from the 'Baozhu' pear was found, purified, and characterized in this report. This chitinase was a monomer with a molecular mass of 28.9 kDa. Results of the internal peptide sequence analyses classify this chitinase as a class III chitinase. In the enzymatic hydrolytic assay, this chitinase could hydrolyze chitin derivatives into di-N-acetylchitobiose (GlcNAc2) as a major product in the initial phase, as well as hydrolyze GlcNAc2 into N-acetylglucosamine (GlcNAc), which represents both chitobiosidase and β-N-acetylglucosaminase activity. Biological analyses showed that this chitinase exhibits strong antifungal activity toward agricultural pathogenic fungi. In total, chitinase from 'Baozhu' pear is a novel bifunctional chitinase that could be a potential fungicide in the biological control of plant diseases.
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