These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.
Pubmed for Handhelds
PUBMED FOR HANDHELDS
Search MEDLINE/PubMed
Title: Analysis of lncRNAs expression in UVB-induced stress responses of melanocytes. Author: Zeng Q, Wang Q, Chen X, Xia K, Tang J, Zhou X, Cheng Y, Chen Y, Huang L, Xiang H, Cao K, Zhou J. Journal: J Dermatol Sci; 2016 Jan; 81(1):53-60. PubMed ID: 26596215. Abstract: BACKGROUND: Long non-coding RNAs (lncRNAs) have close relationships with oxidative stress, nutritional deficiency, DNA damage and other types of cellular stress responses. Previous studies have demonstrated that some non-coding RNAs in melanocytes such as microRNAs can change and contribute to the synthesis of melanin or the development of melanoma after stimulation with UV. However, as an important component of non-coding RNAs, it is unclear what changes occur in lncRNAs during UV-induced stress responses in melanocytes. OBJECTIVE: To explore changes in the expression of long non-coding RNAs (lncRNAs) in melanocytes following UVB-induced stress, and to explore if lncRNAs are involved in the synthesis of melanin. METHODS: Primary melanocytes were irradiated by 20mJ/cm(2) UVB. The MTT method was used to detect cell proliferation. Quantitative real-time PCR was carried out to analyze expression of tyrosinase (TYR) and lncRNAs. Dopa colorimetry was performed to analyze TYR activity. The expression profile of lncRNAs and mRNAs were confirmed using an Agilent Human lncRNA 4×180K chip. Intracellular ROS levels were detected by flow cytometry. ROS scavenger (NAC) was employed to inhibit the ROS level. TYR mRNA expression and activity were re-analysed after transfecting of lnc-CD1D-2:1 siRNA and lnc-SGCG-5:4 siRNA in UVB-irradiated melanocytes to confirm the roles of the two lncRNAs in the synthesis of melanin. phospho-ERK, phospho-p38, and phospho-JNK expressions were detected by Western Blot. RESULTS: Cell proliferation of the 20mJ/cm(2) UVB-irradiated melanocytes decreased to 91% of that of the control cells. Twenty-four hours after irradiation with 20mJ/cm(2) UVB, TYR mRNA expression and activity of the irradiated cells were significantly increased relative to the control group. Chip detection data showed that after irradiation with 20mJ/cm(2) UVB, the expression of 807 lncRNAs and 69 stress response-related genes had changed by more than two-fold. Expression levels of Lnc-GKN2-1:1, lnc-CD1D-2:1, and lnc-SGCG-5:4 and ROS content were significantly increased after UVB irradiation. NAC reduced UVB-induced ROS generation and inhibited UVB-induced upregulation of lnc-GKN2-1:1 and lnc-CD1D-2:1. Lnc-CD1D-2:1 siRNA significantly suppressed the UVB-induced TYR mRNA expression and tyrosinase activation. Lnc-CD1D-2:1 siRNA inhibited UVB-induced p38 phosphorylation. CONCLUSIONS: LncRNAs in melanocytes undergo significant changes following irradiation with 20mJ/cm(2) UVB, suggestting that lncRNAs participate in the UVB-induced stress response. Some lncRNAs expression changes induced by UVB are dependent on ROS generation. ROS-mediated production of lnc-CD1D-2:1 may be involved in the melanogenesis induced by UVB.[Abstract] [Full Text] [Related] [New Search]