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  • Title: 2-DE Gel Analysis: The Spot Detection.
    Author: Martinotti S, Ranzato E.
    Journal: Methods Mol Biol; 2016; 1384():155-64. PubMed ID: 26611414.
    Abstract:
    The abundance of different proteins on a 2-DE gel is reflected by the shape, size, and intensity of the corresponding spots. Protein quantitation requires the conversion of an analog gel image into digital data, resulting into a catalog of individual spots listed as x, y positions, shape parameters, and quantitative values. So, it is possible to carry out objective comparisons of equivalent spots on different gels, determining whether a particular protein is more or less abundant in one sample compared with another. Unfortunately, spots on protein gels are not uniform in shape, size, or density, and detection, quantitation, and comparison can be challenging without intervention. Once a processed image is available, a number of different algorithms can be applied to detect and quantitate individual spots.
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