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  • Title: Detection and Quantification of Protein Spots by Pinnacle.
    Author: Morris JS, Gutstein HB.
    Journal: Methods Mol Biol; 2016; 1384():185-201. PubMed ID: 26611416.
    Abstract:
    Accurate spot detection and quantification is a challenging task that must be performed effectively in order to properly extract the proteomic information from two-dimensional (2-D) gel electrophoresis images. In Morris et al., Bioinformatics 24:529-536, 2008, we introduced Pinnacle, an automatic, fast, effective noncommercial package for spot detection and quantification for 2-D gel images, and subsequently we have developed a freely available gui-based interface for applying the method to a set of gels. In this chapter, we overview Pinnacle, and in a step-by-step manner we describe how to use the software to obtain spot lists and quantifications, to be used for comparative proteomic analysis.
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