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  • Title: Preparation of Escherichia coli elongation factor Tu-guanosine 5'-triphosphate analogs.
    Author: Delaria K, Jurnak F.
    Journal: Anal Biochem; 1989 Feb 15; 177(1):188-93. PubMed ID: 2662816.
    Abstract:
    A simple procedure for the bulk preparation of 20 mg of Escherichia coli elongation factor (EF)-Tu-GTP analogs is described. The protocol is based upon the preparation and stabilization of nucleotide-free EF-Tu using an EF-Ts affinity chromatographic resin. The procedure is a general one for the preparation of any GTP analog of EF-Tu.
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