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  • Title: Purification and characterization of cytokine-induced neutrophil chemoattractant produced by epithelioid cell line of normal rat kidney (NRK-52E cell).
    Author: Watanabe K, Kinoshita S, Nakagawa H.
    Journal: Biochem Biophys Res Commun; 1989 Jun 30; 161(3):1093-9. PubMed ID: 2662972.
    Abstract:
    Interleukin-1 beta, tumor necrosis factor-alpha and lipopolysaccharide stimulated normal rat kidney cell line (NRK-52E) to produce a chemotactic factor for rat neutrophils. This cytokine-induced neutrophil chemoattractant (CINC) was purified to obtain a single band with a M.W. of 63,000 on SDS-PAGE. The purified CINC induced detectable migration and strong chemotaxis of neutrophils at concentrations of 10(-9)M and 10(-7) - 10(-8)M, respectively. Checkerboard analysis indicated that CINC was a real chemotactic factor. Amino acid composition of CINC showed that CINC has a resemblance to human monocyte-derived neutrophil chemotactic factor (MDNCF) rather than human complement fragment, C5a.
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